精品久久人人妻人人做精品,伊人色啪啪天天综合婷婷,国语自产少妇精品视频蜜桃在线,精品欧美一区二区在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>COLO-320人結(jié)腸腺癌細(xì)胞

人結(jié)腸腺癌細(xì)胞

簡(jiǎn)要描述:COLO-320 人結(jié)腸腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):COLO-320
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-06-23
  • 訪  問(wèn)  量:1779

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

COLO-320 人結(jié)腸腺癌細(xì)胞

ATCC® Number: CCL-220™

Designations: COLO 320DM

Depositors: GE Moore

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens (human)

Morphology: rounded and refractile

COLO-320 人結(jié)腸腺癌細(xì)胞

Source: Organ: colon

Tumor Stage: Dukes' type C

Disease: colorectal adenocarcinoma

Cellular Products: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Cytogenetic Analysis: modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Isoenzymes: ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

Add 2.0 to 3.0 of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products

COLO-320


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
性欧美XXXXX老少交| 国产伦精品一区二区三区不卡| 国产AV天堂一区二区三区| 国产精品久久久久久精品电影| 人妻日本无中文字幕无码| 久久AV无码专区亚洲AV桃花岛| 日本55丰满熟妇厨房伦| 一本一道VS无码中文字幕| 无码人妻丰满熟妇A片护士| 被黑人猛躁10次高潮视频| 被几个领导玩弄一晚上| 东南亚11孩岁女精品A片| 国产精品久久一区二区| 中文字幕乱码中文字乱码| 人妻 日韩精品 中文字幕 | 国产AV无码专区亚洲版综合| 国产强伦姧在线观看无码| 各种少妇WBB撒尿| 亚洲日韩欧美一区久久久久我| 亚洲暴爽AV天天爽日日碰| 免费成人在线视频| 免费播放欧美毛片欧美AAAAA| А√天堂资源官网在线资源| 色婷婷狠狠久久综合五月| 超模的秘密在线观看| 久久亚洲精品中文字幕| 国产GV猛男GV无码男同网站| 成人乱人乱一区二区三区| 我和两个小婕子做过爱| 把女人弄爽特黄A大片视频| 国产精品久久久久久久久爆乳| 亚洲午夜精品久久久久久APP| 免费真人视频APP| 国产精品爱久久久久久久小说| 美女做爰A片毛片AAAA| 一本加勒比HEZYO东京热高清| 护士被两个病人伦奷日出白浆| 老头把舌头伸进粉嫩淑芬| AV无码AV高潮AV喷吹免费| 精品久久久久久亚洲精品| 8X8×拨牐拨牐永久免费视频|