精品久久人人妻人人做精品,伊人色啪啪天天综合婷婷,国语自产少妇精品视频蜜桃在线,精品欧美一区二区在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1504ZR-75-30 人乳腺癌細(xì)胞

ZR-75-30 人乳腺癌細(xì)胞

簡要描述:CRL-1504 ZR-75-30 人乳腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1504
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1934

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1504  ZR-75-30 人乳腺癌細(xì)胞

ATCC® Number:CRL-1504™Price:
Designations:ZR-75-30Depositors:LW EngelBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: mammary gland; breastDisease: ductal carcinomaDerived from metastatic site: ascitesCell Type: epithelialPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-1504  ZR-75-30 人乳腺癌細(xì)胞DNA Profile (STR):Amelogenin: XCSF1PO: 10,11D13S317: 10,11D16S539: 9D5S818: 12,13D7S820: 11,12THO1: 8,9TPOX: 10,11vWA: 18,19Cytogenetic Analysis:This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 40% of cells. The rate of cells with higher ploidies was 1.2%. Sixteen marker chromosomes were found in most cells.Age:47 yearsGender:femaleEthnicity:BlackComments:ZR-75-30 was derived from malignant ascites fluid from a 47-year-old premenopausal Black woman with infiltrating ductal carcinoma. These cells have been characterized to be human, non-HeLa, malignant mammary epithelium in origin.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommendedMedium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseDoubling Time:110 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020purified DNA:ATCC 45532purified DNA:ATCC 45533purified DNA:ATCC CRL-1504DReferences:1230: Engel LW, Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978. PubMed: 21219323031: Engel LW, et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978. PubMed: 68822532275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764


CRL-1504  


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
CAOPORN免费视频国产| 久久精品中文字幕一区二区三区| 久久精品无码人妻A级毛片| 国产情侣疯狂作爱系列| 久久99精品久久久久久无毒不卡| A片在线免费观看| 美女脱内衣让男生揉摸的视频| 欧美熟妇浓毛大BBW| 国产精品久久久久久久久免费| 亚洲精品无码AV人在线观看国产 | 久久久无码精品亚洲日韩| 天天摸夜夜添添到高潮水汪汪| 精品无码国产一区二区三区麻豆| 天天摸天天做天天爽天天弄| YING荡的雯雯第三部分| 荡乳尤物H窑子开张了| 在厨房拨开内裤进入毛片| 我和小娻孑在卧室做了| 青青青国产精品一区二区| 少妇饥渴偷公乱400章深夜书屋| 最近国语视频在线观看免费播放| 97无码精品人妻一区二区| 69麻豆天美精东蜜桃传媒| 欧美交A欧美精品喷水| 亚洲精品无码久久久久久久| 日本熟妇乱人伦A片免费高清| 精品人妻少妇嫩草AV无码专区| 免费看国产曰批40分钟| 含紧一点H边做边走| 国产 AV 仑乱内谢| 性妇BBW搡BBBB搡BBBB| 欧美精品毛片久久久久久久| 亚洲A∨无码一区二区三区 | 扒开女人内裤猛进猛出免费视频| 娇妻在客厅被朋友玩得呻吟| 久久久久亚洲精品中文字幕| 狂躁女人双腿流白色液体| 国产在线国偷精品产拍| 免费看男男GAY啪啪网站| 九九久久精品无码专区| 夜夜躁天天躁很躁|